the discovery of small-molecule mimicking peptides through phage display小分子的发现模仿通过噬菌体展示肽.pdf

Molecules 2011, 16, 774-789; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review The Discovery of Small-Molecule Mimicking Peptides through Phage Display Fahriye Ceyda Dudak 1, Ismail Hakki Boyaci 1 and Brendan P. Orner 2,* 1 Department of Food Engineering, Hacettepe University, Beytepe 06800, Ankara, Turkey; E-Mails: ceyda@.tr (F.C.D.); ihb@.tr (I.H.B.) 2 Division of Chemistry and Biological Chemistry, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore * Author to whom correspondence should be addressed; E-Mail: orner@.sg; Tel.: +65-6316-8757;Fax: +65-6791-1961. Received: 16 December 2010; in revised form: 12 January 2011 / Accepted: 18 January 2011 / Published: 19 January 2011 Abstract: Using peptides to achieve the functional and structural mimicry of small- molecules, especially those with biological activity or clear biotechnological applications, has great potential in overcoming difficulties associated with synthesis, or unfavorable physical properties. Combinatorial techniques like phage display can aid in the discovery of these peptides even if their mechanism of mimicry is not rationally obvious.The major focus of this field has been limited to developing biotin and sugar mimetics. However, the full “mimicry” of these peptides has not yet been fully established as some bind to the target with a different mechanism than that of the natural ligand and some do not share all