Western印迹杂交分析p53蛋白在人鼻咽癌中的表达.pdf

18 ( A cad J SUMS) , 1999, 20( ) : 18~ 20 Western 印迹杂交分析p53 蛋白在人鼻咽癌中的表达 ( ; , 510060) : p53 : Western , p53 : 104 , 12 ( 115% ) p53 ; 8 , p53 IgA/ VCA 1160 p53 118% ( 11/ 93) , IgA/ VCA 180 , p53 91% ( 1/ 11) IgA / EA , p53 141% ( 11/ 78) , IgA/ EA , p53 38% ( 1/ 26) p53 , 2 : p53 , , IgA/ VCA IgA/ EA , p53 ; , p53; , R 739 63 Western Blot Analysis of p53 ProteinExpressioninHuman Nasopharyngeal Carcinoma Zheng Meilian Ou Baoxiang Long Jianbin Deng Manquan Chen Lizhen Liang Q iw an Li Hu imei ( Cancer Institute, Sun Yatsen University of Medical Sciences, Guangzhou, 510060) Abstract Objective:To investigate the expression of p53 protein in nasopharyngeal carcinoma and its clinical sig nificant ethods: Western blot hybridizat ion and immunopercipitat ion techniques w ere used to examine protein expres sion of p53 gene in human nasopharyngeal biopsy Results:The 104 nasopharyngeal carcinoma biopsy 12 ( 11 5% ) showed abnormal expression of p53 prot ein Abnormal expression of p53 protein was not found in 8 biopsy of the na sopharyngeal chronic inflammation The protein abnormal of p53 gene w ere 11 8% ( 11/ 93) and 91% ( 1/ 11) in na sopharyngeal carcinoma w ith EBVIgA/ VCA 1160 and EBVIgA/ VCA 180, respectively The p53 protein positive rates of mutationtype w ere 141% ( 11/ 78) and 38% ( 1/ 26) , respect ively in nasopharyngeal carcinoma w ith EBV IgA/ EA positive and negative 2 patients w as found cancer recurrence in 12 pat ients of p53 protein abnormal expression Conclussion:The study show ed that the p53 protein abnormal expression in patients w as frequent in higher malignant de gree, later stage, higher IgA/ VCA antibody titre and IgA/ EA seropositive It su