高效液相色谱法测定蔬菜中8种氨基甲酸酯类农药残留_邵金良.pdf

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.7 高效液相色谱法测定蔬菜中8种氨基甲酸酯类农药残留 邵金良，黎其万，刘宏程，佴注，兰珊珊，梅文泉，杜丽娟，樊建麟 （云南省农业科学院质量标准与检测技术研究所，云南昆明 650223） 摘要：建立了固相萃取- 高效液相色谱柱后衍生荧光检测蔬菜中涕灭威亚砜，涕灭威砜，灭多威，三羟基克百威，涕灭威，克 百威，甲萘威及异丙威等8种氨基甲酸脂类农药残留的分析方法。本文对测定氨基甲酸酯农药残留的分离条件，提取溶剂，洗脱溶 剂，净化材料和基质效应进行了优化试验。结果显示，8种氨基甲酸脂类农药在0.1~1.0 mg/L的浓度范围内线性良好，线性相关系数 为0.99943~0.99992 ，检出限为0.008~0.01 mg/kg之间。样品经乙腈提取，氨基小柱净化，采用基质加标，在0.1mg/kg添加水平的平 均回收率为79.4%~119.0% ，相对标准偏差2.5%~11.5% ；0.2 mg/kg 添加水平的平均回收率为81.2%~115.5% ，相对标准偏差为 2.8%~8.1% 。此方法可适用于大白菜，辣椒，茄子，番茄，菠菜，苦瓜，结球甘蓝和豇豆等样品的氨基甲酸酯类农药残留的分析测 定。 关键词：高效液相色谱法；柱后衍生；氨基甲酸酯类农药；蔬菜 文章篇号：1673-9078(2011)7-856-860 Determination of Eight Carbamate Pesticides in Vegetables by High Performance Liquid Chromatography SHAO Jin-Liang, LI Qi-Wan, LIU Hong-cheng, ER-Zhu, LAN Shan-shan MEI Wen-quan, DU Li-juan, FAN Jian-lin (Institute of Agriculture Quality standards＆Testing Technique, Yunnan Academy of Agricultural Science, Kunming, Yunnan 650223, China) Abstract: A new method for the simultaneous determination of eight carbamate pesticides in Chinese cabbage, pepper, egg-plant, tomato, Chinese spinach, bitter gourd, cabbage and cowpea by high performance liquid chromatography was developed. Eight carbamate pesticides was extracted from the samples by acetonitrile, salted, purified with LC-NH2 column and determined by high performance liquid chromatography using post-column derivatization with fluorescence detector. The proposed methods showed a good linearity between analytical signal in the range of 0.1~1.0 mg/L with the linea