急性呼吸窘迫综合征的影响.docx

过热对脂多糖介导的大鼠急性肺损伤/急性呼吸窘迫综合征的影响 作者：林晓静，邹飞，李亚洁，王斌，韩雪琳，贺文静 【关键词】 急性肺损伤 Aggravating effect of hyperthermia on lipopolysaccharidemediated ALI/ARDS in rats 　　【Abstract】 AIM: To observe the effect of hyperthermia on lipopolysaccharide (LPS)mediated ALI/ARDS in rats. METHODS: Male specificpathogen free Wistar rats were randomly assigned to the following groups: salineinjected normothermic control (CGroup), salineinjected heat exposed (HGroup), LPSinjected normothermic control (LGroup) and LPSinjected heat exposed (HLGroup). Rectal temperature (Tr) was continually monitored. Plasma levels of TNFα and IL6, arterial blood gas, lung wettodry weight ratio (W/D), and lung histological change were observed at 0, 40, 80 and 120 min. RESULTS: ① The rats in HLGroup displayed significantly higher values of Tr (43.04±0.11) ℃ compared with C/LGroup （Plt;0.01）. There was no significant difference between HLGroup and HGroup （Pgt;0.05）. ② Significant elevations of the peak TNFα level (at 80 min) and IL6 expression were seen in HLGroup （Plt;0.01）. ③ The values of HCO3 and PaCO2 significantly decreased at 40 min in HLGroup（Plt;0.01）. At 120 min, the values of HCO3 and PaCO2 decreased to (10.42±1.06) mmol/L and (2.82±0.81) kPa respectively. ④ Significantly increased W/D ratio and many pathological features of acute microvascular lung injury were seen in HLGroup. CONCLUSION: Hyperthermia may aggravate ALI/ARDS in LPSinjected rats.　　【Keywords】 hyperthermia; LPS; ALI/ARDS; rats　　【摘要】 目的： 研究过热对脂多糖（LPS）介导的大鼠急性肺损伤/急性呼吸窘迫综合征（ALI/ARDS）的影响. 方法： 雄性SPF级Wistar大鼠随机分为： 常温生理盐水组（C组），高温生理盐水组（H组），常温脂多糖组（L组），高温脂多糖组（HL组）. 持续描计动物直肠温度（Tr），并检测动物应激0， 40， 80， 120 min时血浆TNFα， IL6含量，动脉血气，血浆肺湿质量/干质量比值（W/D）以及肺病理改变. 结果： ① HL组Tr上升到（43.04±0.11）℃，与C组，L组之间存在显著性差异（Plt;0.01），与H组之间不存在显著性差异（Pgt;0.05）；② HL组动物血浆TNFα峰值（80 min）及 IL6表达水平显著升高（Plt;0.01）；③ HL组HCO3-，PaCO2于致伤40 min时即出现显著下降（Plt;0.01），致伤120 min时分别为（10.42±1.06） mmol/L， （2.82±0.81） kPa；④ HL组动物W/D显著性升高（Plt;0.05）；肺急性微血管损伤有病理学改变. 结论： 过热应激能够加重LPS介导的大鼠ALI/ARDS.