type i interferon reaction to viral infection in interferon-competent, immortalized cell lines from the african fruit bat eidolon helvum在interferon-competent i型干扰素对病毒感染的反应,来自非洲果蝠的永生化细胞系的精灵helvum.pdf

Type I Interferon Reaction to Viral Infection in Interferon- Competent, Immortalized Cell Lines from the African Fruit Bat Eidolon helvum 1 1 1,2 1 1 Susanne E. Biesold , Daniel Ritz , Florian Gloza-Rausch , Robert Wollny , Jan Felix Drexler , Victor M. 1 3,4 5 1 ¨ 1 Corman , Elisabeth K. V. Kalko , Samuel Oppong , Christian Drosten *, Marcel A. Muller 1 Institute of Virology, University of Bonn Medical Centre, Bonn, Germany, 2 Noctalis, Centre for Bat Protection and Information, Bad Segeberg, Germany, 3 Institute of Experimental Ecology, University of Ulm, Ulm, Germany, 4 Smithsonian Tropical Research Institute, Balboa, Panama, 5 Kwame Nkrumah University of Science and Technology, Kumasi, Ghana Abstract Bats harbor several highly pathogenic zoonotic viruses including Rabies, Marburg, and henipaviruses, without overt clinical symptoms in the animals. It has been suspected that bats might have evolved particularly effective mechanisms to suppress viral replication. Here, we investigated interferon (IFN) response, -induction, -secretion and -signaling in epithelial-like cells of the relevant and abundant African fruit bat species, Eidolon helvum (E. helvum). Immortalized cell lines were generated; their potential to induce and react on IFN was confirmed, and biological assays were adapted to application in bat cell cultures, enabling comparison of landmark IFN properties with that of common mammalian cell lines. E. helvum cells were fully capable of reacting to viral and artificial IFN stimuli. E. helvum cells showed highest IFN mRNA inductio