姜黄素对大鼠肺微血管平滑肌细胞增殖的影响.doc

姜黄素对大鼠肺微血管平滑肌细胞增殖的影响 复旦大学附属中山医院麻醉科 邮编200032 张鹏 黄海琼 姜桢 【摘要】目的：研究姜黄素对大鼠肺微血管平滑肌细胞增殖的影响。方法：实验分对照组和不同浓度姜黄素处理组。药物作用24小时后，MTT法测姜黄素对大鼠肺微血管平滑肌细胞增殖的抑制作用，流式细胞仪检测姜黄素作用后细胞周期变化。结果：姜黄素以浓度依赖的方式抑制肺微血管平滑肌细胞增殖；流式细胞仪分析显示，姜黄素处理过的细胞S期明显减少，主要停滞在G0/G1期。结论：姜黄素可以抑制大鼠肺微血管平滑肌细胞增殖，呈现浓度依赖性，其作用可能是通过阻滞细胞周期进程实现的。 关键词：姜黄素；肺小血管平滑肌细胞；细胞周期 Effect of curcumin on proliferation of rat pulmonary microvascular smooth muscle cells 【Abstract】Aim: To explore the effect of curcumin on proliferation of rat pulmonary microvascular smooth muscle cells. Methods: The inhibitory rate of rat pulmonary microvascular smooth cells in different concentrations of curcumin were measured by MTT assay. Cell cycle was detected by flow cytometry. Result: The inhibitory effect of curcumin on rat pulmonary microvascular smooth cells was showed in a dose-dependent manner(P﹤0.05). Cell cycle analysis showed reduced percentage of cells in S phase and G0/G1 phase arrest(P﹤0.05). Conclusion: Curcumin exerts inhibitory effect on proliferation of rat pulmonary microvascular smooth muscle cells in a dose-dependent manner possibly through induing cell cycle arrest. Key words: curcumin; pulmonary microvascular smooth muscle cells; cell cycle 肺动脉高压发病中病理学改变主要在肺小动脉 ADDIN EN.CITE EndNoteCiteAuthorDavies/AuthorYear1991/YearRecNum24/RecNumrecordrec-number24/rec-numberforeign-keyskey app=EN db-id=d5sfez0sp0px27ex0rl5p0909ppvzrpxve9v24/key/foreign-keysref-type name=Journal Article17/ref-typecontributorsauthorsauthorDavies, P./authorauthorReid, L./author/authors/contributorsauth-addressDepartment of Pathology, Childrenapos;s Hospital, Boston, Massachusetts./auth-addresstitlestitleHypoxic remodeling of the rat pulmonary arterial microcirculation assessed by microdissection/titlesecondary-titleJ Appl Physiol/secondary-title/titlesperiodicalfull-titleJ Appl Physiol/full-title/periodicalpages1886-91/pagesvolume71/volumenumber5/numberedition1991/11/01/editionkeywordskeywordAnimals/keywordkeywordAnoxia/complications/*pathology/keywordkeywordDissection/