发酵豆粕中大豆异黄酮提取条件的优化.pdf

发酵豆粕中大豆异黄酮提取条件的优化 孙洁心1，孙强1，张永忠2‘3·’ (1．黑龙江农垦职业学院，黑龙江哈尔滨150025；2东北农业大学应用化学系，黑龙江哈尔滨150030； 3．教育部大豆生物学重点实验室，黑龙江哈尔滨150030) 摘要：旨在优化发酵豆粕中大豆异黄酮的提取条件，通过单因素试验，确定从发酵豆粕中提取大豆异黄酮的最佳提 取条件是：60％乙醇，料液比2009／L，室温下提取60min，提取2次。此条件下异黄酮提取总量为2855．485pg／g。 关键词：提取；发酵豆粕；大豆异黄酮 Proce鹦Conditio璐of Isonav仰esfromFe珊ented Meal of Soybean OptiIIlization Extracting Soybean suN Jie—xinl，suN Yong—zhon孑3+ Qian91，zHANG Vocational Chemistry， 150025，Heilon西iang，china；2．DepartmentofAPplied (1．Heilon舀iangNongken College，Harbin Northeast of of LaboratorySoybeaJlBiology，Ministry A鲥culturalUniversity，Harbin150030，Heilon自iang，China；3．Key 1 Education，Harbin 50030，Heilon西iang，China) of fe姗ented aimsto theextractionconditionsisonavoneshom Abstract：This soybean． paper optimize the ofisonaVonesfrom tothes010factors isascertainedthat extraction conditions According test，it technological wasthe ofethanol ratio200 mom fermentedresidue 60％，the soybean pIDponion solid—liquid g，IJthe 2 tot础time60minandextractiontwice．The ofjsonaVoneswas855．485 tempeJIature，the grossproduct ILg／夸with