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何首乌茎叶总黄酮的提取及其抗运动疲劳作用.PDF

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2017 年 2 月 食品研究与开发 第 38 卷第 3 期 Food Research And Development 分离提取 66 DOI :10.3969/j .issn.1005-6521.2017.03.015 何首乌茎叶总黄酮的提取及其抗运动疲劳作用 1 2 ,* 钱小东 ,黄伟 (1.湖北第二师范学院 体育学院 ,湖北 武汉 430205 ;2. 湖北省肿瘤医院 妇瘤科 ,湖北 武汉 430079 ) 摘 要 :利用单 因素和正交试验对超声波乙醇浸提法提取何首乌茎叶总黄酮的工艺进行优化 ,并对何首乌茎叶总黄酮 的抗运动疲劳作用进行研究。结果表明,超声波乙醇浸提法提取何首乌茎叶总黄酮的最佳提取工艺为:超声时间 40 min ,乙 醇浓度 70 % ,料液比 1 ∶ 40(g/mL ),提取温度 80 ℃ ,在此工艺条件下何首乌茎叶总黄酮的得率可达 2.49 %。小鼠跑 台力 竭 实验和 负重游泳实验表明何首乌茎叶总黄酮具有 良好的抗运动疲劳能力。 关键词 : 何首乌茎叶;总黄酮;超声波乙醇浸提 ;正交试验 ;抗运动疲劳 Extraction and Anti-exercise-fatigue Activity of Flavonoids from Stalks and Leaves of Polygonum multif lorum thunb 1 2,* QIAN Xiao-dong , HUANG Wei (1. Department of Physical Education , Hubei University of Education , Wuhan 430205 , Hubei , China ; 2. Department of Female Tumor , Hubei Province Tumor Hospital , Wuhan 430079 , Hubei , China ) Abstract : The ultrasonic and ethanol immersion extraction process of flavonoids from stalks and leaves of Polygonum multif lorum thunb (FSLPMT )was optimized using the single factor experiment and orthogonal ex- periment. In addition , the anti -exercise -fatigue activity of FSLPMT was studied. The results showed that the optimum extraction condition of FSLPMT using ultrasonic and ethanol immersion extraction method was : ultra- sonic extraction time 40 min , volume fraction of ethanol 70 % , liquid material ratio 1 ∶ 40 (g/mL ), and ultra- sonic extraction temperature 80 ℃ . Under the optimum conditions , the obtained rate of FSLPMT was 2.49 % . The results of the treadmill test and weight-lo
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