转化生长因子β1对钛表面成骨细胞因子表达的影响（毕业论文）.doc

转化生长因子β1对钛表面成骨细胞因子表达的影响 【摘要】目的：通过观测特定浓度的转化生长因子β1（transforming growth factors β1,TGF-β1）对钛表面成骨细胞中骨钙素(osteocalcin,OC)、骨涎蛋白(bone sialoprotein,BSP)和I型胶原蛋白(collagen-I,COL-I)表达的影响，以探讨其对钛表面成骨细胞增殖分化的作用。方法：体外培养成骨细胞接种于钛片表面，将其分为刺激组和对照组，刺激组加入0.5ng/ml的TGF-β1刺激细胞增殖和分化，收集第1d、3d、7d培养的细胞。通过RT-PCR检测不同培养时间OCmRNA、BSPmRNA和Col-ImRNA表达情况，对照组不加TGF-β1。实验中所有数据采用SPSS 10.0软件作统计学处理分析，比较各组间差异程度，P 0.05认为差异有显著性意义。结果：浓度为0.5ng/ml的外源性TGF-β1加入到接种于钛片表面的成骨细胞中后，均可显著增加几种细胞因子（OC、BSP和Col-I）的表达（P0.05）。结论：浓度为0.5ng/ml的外源性TGF-β1对钛片表面的成骨细胞成骨相关因子表达有一定的促进作用。 关键词：TGF-β1；钛；成骨细胞；RT-PCR [中国图书分类号] [文献标识码] [文章编号] The effects of TGF-β1 on the gene expression of osteoblasts on Titanium disc surfaces in vitro ZHANG An-mei (Yinan county peoples hospital, LinYi ShanDong 276300,China) [Abstract]Objective: The purpose of this study is to investigate the consequential changes of gene expression of osteoblasts on Titanium disc surfaces incubated with TGF-β1,and to make a primary understanding of functions of proliferation and differentiation.Methods: Osteoblast were divided into two groups: the control group and the induced group. The cells were seeded on surfaces of Ti disc and composed of Dexamethasone(DEX), β-phosphoglycerol and Vitamin C. TGF-β1 with a dose of 0.5ng/ml was administrated to induced group.The expression of mRNA were detected by reverse transcription-polymerase chain reaction(RT-PCR).All the data were analyzed by SPSS10.0 software.Results:The extraneous TGF-β1 (0.5ng/ml) can increase the gene expressions（OC,BSP and Col-I） of osteoblasts on Ti disc surfaces（P0.05）.Conclusions:It is concluded that extraneous TGF-β1 with a dose of 0.5ng/ml increased osteoblase gene expressions on the surfaces of Ti disc. Key word：TGF-β1;Ti disc;Osteoblast; RT-PCR 张安美 沂南县人民医院口腔科副主任 主治医师 山东 沂南 276300 [作者简介] 张安美（1970-06），女，沂南县人民医院口腔科副主任，主治医师 E-mail：HYPERLINK mailto:zhengkouqiang@163.co126.com，Tel中国社会渐步入老龄化，牙体缺失情况越来越严重，随着经济水平的增长及对美观要求的提高，种植作为修复缺失牙的重要手段变得越来越重要。种植成功的关键在于形成良好的种植体-骨界面