testing a short nuclear marker for inferring staphylinid beetle diversity in an african tropical rain forest测试一个短核标记推断staphylinid甲虫在非洲热带雨林多样性.pdf

Testing a Short Nuclear Marker for Inferring Staphylinid Beetle Diversity in an African Tropical Rain Forest 1 2 3 ¨ 1 4 Birthe Thormann *, Michael J. Raupach , Thomas Wagner , Johann W. Wagele , Marcell K. Peters 1 Zoological Research Museum Alexander Koenig, Bonn, Germany, 2 German Centre for Marine Biodiversity Research, Research Institute Senckenberg, Wilhelmshaven, ¨ ¨ Germany, 3 Abteilung Biologie, Institut fur Integrierte Naturwissenschaften, Universitat Koblenz – Landau, Koblenz, Germany, 4 Department of Animal Ecology and ¨ ¨ Tropical Biology, Biocenter, University of Wurzburg, Am Hubland, Wurzburg, Germany Abstract Background: The use of DNA based methods for assessing biodiversity has become increasingly common during the last years. Especially in speciose biomes as tropical rain forests and/or in hyperdiverse or understudied taxa they may efficiently complement morphological approaches. The most successful molecular approach in this field is DNA barcoding based on cytochrome c oxidase I (COI) marker, but other markers are used as well. Whereas most studies aim at identifying or describing species, there are only few attempts to use DNA markers for inventorying all animal species found in environmental samples to describe variations of biodiversity patterns. Methodology/Principal Findings: In this study, an analysis of the nuclear D3 region of the 28S rRNA gene to delimit species-like units is compared to results based on distinction of morphospecies. Data derived from bot