a 3d model of the membrane protein complex formed by the white spot syndrome virus structural proteins膜蛋白的三维模型形成的复杂的白斑综合症病毒结构蛋白.pdf

A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins 1 . 2. 3. 1 1 Yun-Shiang Chang * , Wang-Jing Liu , Cheng-Chung Lee , Tsung-Lu Chou , Yuan-Ting Lee , Tz-Shian 1 2 1 1 2 3 Wu , Jiun-Yan Huang , Wei-Tung Huang , Tai-Lin Lee , Guang-Hsiung Kou , Andrew H.-J. Wang *, Chu- Fang Lo2* 1 Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan, 2 Institute of Zoology, National Taiwan University, Taipei, Taiwan, 3 Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan Abstract Background: Outbreaks of white spot disease have had a large negative economic impact on cultured shrimp worldwide. However, the pathogenesis of the causative virus, WSSV (whit spot syndrome virus), is not yet well understood. WSSV is a large enveloped virus. The WSSV virion has three structural layers surrounding its core DNA: an outer envelope, a tegument and a nucleocapsid. In this study, we investigated the protein-protein interactions of the major WSSV structural proteins, including several envelope and tegument proteins that are known to be involved in the infection process. Principal Findings: In the present report, we used coimmunoprecipitation and yeast two-hybrid assays to elucidate and/or confirm all the interactions that occur among the WSSV structural (envelope and tegument) proteins VP51A, VP19, VP24, VP26 and VP28. We found that VP51A interacted directly not only with VP26 but also with VP19 and VP24. VP51A, VP19 and VP24 were also shown to have an affinity for self-interaction. Chemical cross-linking assays sh