高效毛细管电泳法测定鸡蛋中环丙沙星的研究.pdf

, Journal of AnhuiA gri. S .i 2010, 38( 36) : 20717- 20719 李菲菲 马君叶 * 周莹, 周梅仙 (, 211171) [目的]建立高效毛细管电泳测定鸡蛋中环丙沙星含量的方法[方法]通过对电泳 冲液类型离子浓度和 pH 值样品溶剂种 类分离电压分离温度和冲洗程序等电泳条件进行考察, 确立测定鸡蛋中环丙沙星的高效毛细管电泳法, 并测定电泳参数对检测结果 的影响[结果]在检测波长为 277 nm 时, 最佳电泳条件为: 电泳 冲液为 30mmol/L N a B O - 10mmol/L KH PO 溶液( pH值 8. 53), 2 4 7 2 4 未涂层石英毛细管( 50 m 47 m, 有效长度 38. 5 m )为分离通道, 分离电压为 18 kV在鸡蛋中对环丙沙星的检测结果显示, 在最佳电 泳条件下, 环丙沙星在 11m in内实现基线分离, 在 0. 25~ 8000 g/m l浓度范围内其浓度和峰面积呈良好的线性关系(R 2 = 0. 999 2), 平 均加标回收率为 101. 48% , 平均RSD 为 2. 466 4% (n = 5)[结论]该方法快速简便灵敏度高重现性好, 可用于鸡蛋中环丙沙星含量 的测定 毛细管电泳; 环丙沙星; 鸡蛋 S 831 A 0517- 6611( 2010) 36- 20717- 03 Study on High Performance CapillaryElectrophoresis forDetermination of Ciprofloxacin in Egg ZHOU Ying et al ( College of Chem i al and Env ironm ental Eng ineer ing ofN anjing X iaozhuang College, N anjing, Jiangsu 211171) A bstra t Abstract [ Obje tive] The study ami ed to estab lish am ethod of determ ining the iprofloxa in in egg by the high performan e apil lary ele trophoresis( HPCE). [M ethod] Through the exam ination on the ele trophoresis onditions su h as the ele trophoresis buffer types, ion on n. and pH value, samp le solvent k inds, separation voltage, separating temperature and washing pro edure, the HPCE m ethod for de term ining the iprofloxa in in egg were established and the effe ts of the ele trophoreti parameters on the testing results w ere determ ined. [ R esu lt] W hen the dete tion wavelength w as set at 277 nm, the optmi um ele trophoreti onditionsw ere as follows: the ele trophoreti buffer was 30 mmol/L N a B O - 10 mmol/L KH PO solution ( pH 8. 53), the non oating quartz apillary ( 50 m 47 m, effe tive length of 2 4 7 2 4 38. 5 m) w as as the separating hanne,l th