the generation of live offspring from vitrified oocytes代的生活玻化卵母细胞的后代.pdf

The Generation of Live Offspring from Vitrified Oocytes 1,2 1,3 1 4 5 L. Gabriel Sanchez-Partida , Richard D. W. Kelly , Huseyin Sumer , Camden Y. Lo , Rotem Aharon , 6 7 1,3 Michael K. Holland , Moira K. O’Bryan , Justin C. St. John * 1 Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Victoria, Australia, 2 Australian Phenomics Network, Monash Animal Research Platform, Monash University, Victoria, Australia, 3 Mitochondrial and Reproductive Genetics Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry, United Kingdom, 4 School of Mathematical Sciences, Monash University, Victoria, Australia, 5 Monash Micro Imaging – MHTP, Monash Institute of Medical Research, Clayton, Victoria, Australia, 6 School of Veterinary Sciences, The University of Queensland, St. Lucia Campus, Brisbane, Queensland, Australia, 7 The Department of Anatomy and Developmental Biology, Monash University, Victoria, Australia Abstract Oocyte cryopreservation is extremely beneficial for assisted reproductive technologies, the treatment of infertility and biotechnology and offers a viable alternative to embryo freezing and ovarian grafting approaches for the generation of embryonic stem cells and live offspring. It also offers the potential to store oocytes to rescue endangered species by somatic cell nuclear transfer and for the generation of embryonic stem cells to study development in these species. We vitrified mouse oocytes using a range of concentrations of trehalose (0 to 0.3 M) and demonstrated tha