cytotoxic effects of cdse quantum dots on maturation of mouse oocytes, fertilization, and fetal developmentcdse量子点的细胞毒性的影响小鼠卵母细胞的成熟,受精和胎儿发育.pdf

Int. J. Mol. Sci. 2009, 10, 2122-2135; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Cytotoxic Effects of CdSe Quantum Dots on Maturation of Mouse Oocytes, Fertilization, and Fetal Development Ming-Shu Hsieh, Nion-Heng Shiao and Wen-Hsiung Chan * Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan * Author to whom correspondence should be addressed; E-Mail: whchan@.tw Received: 3 February 2009; in revised form: 26 April 2009 / Accepted: 28 April 2009 / Published: 14 May 2009 Abstract: Quantum dots (QDs) are useful novel luminescent markers, but their embryonic toxicity is yet to be fully established, particularly in oocyte maturation and sperm fertilization. Earlier experiments by our group show that CdSe-core QDs have cytotoxic effects on mouse blastocysts and are associated with defects in subsequent development. Here, we further investigate the influence of CdSe-core QDs on oocyte maturation, fertilization, and subsequent pre- and postimplantation development. CdSe-core QDs induced a significant reduction in the rates of oocyte maturation, fertilization, and in vitro embryo development, but not ZnS-coated CdSe QDs. Treatment of oocytes with 500 nM CdSe-core QDs during in vitro maturation (IVM) led to increased re