the bradykinin bk2 receptor mediates angiotensin ii receptor type 2 stimulated rat duodenal mucosal alkaline secretion缓激肽bk2受体介导血管紧张素ⅱ2型受体刺激大鼠十二指肠粘膜碱性分泌物.pdf

BMC Physiology BioMed Central Research article Open Access The bradykinin BK2 receptor mediates angiotensin II receptor type 2 stimulated rat duodenal mucosal alkaline secretion Sara Ewert*, Berndt Johansson, Mathias Holm, Herbert F Helander and Lars Fandriks Address: Department of Gastrosurgical Research, Sahlgrenska Academy, Goteborg University, Gothenburg, Sweden Email: Sara Ewert* - sara.ewert@gastro.gu.se; Berndt Johansson - berndt.johansson@gastro.gu.se; Mathias Holm - mathias.holm@gastro.gu.se; Herbert F Helander - herberthelander@; Lars Fandriks - lars.fandriks@gastro.gu.se * Corresponding author Published: 22 February 2003 Received: 27 September 2002 Accepted: 22 February 2003 BMC Physiology 2003, 3:1 This article is available from: /1472-6793/3/1 © 2003 Ewert et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: This study investigates bradykinin and nitric oxide as potential mediators of AT2- receptor-stimulated duodenal mucosal alkaline secretion. Duodenal mucosal alkaline secretion was measured in methohexital- and α-chloralose-anaesthetised rats by means of in situ pH-stat titration. Immunohistochemistry and Western blot were used to identify the BK2 receptors. Results: The AT2 receptor agonist CGP42112A (0.1 µg kg-1 min-1) administered intravenously increased the duod