bronchus-associated lymphoid tissue (balt) and survival in a vaccine mouse model of tularemia此外淋巴组织(balt)和生存在兔热病的疫苗小鼠模型.pdf

Bronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemia 1 2 1 1 Damiana Chiavolini , Javier Rangel-Moreno , Gretchen Berg , Kate Christian , Laura Oliveira- 1,3 1 4 2 1 Nascimento , Susan Weir , Joseph Alroy , Troy D. Randall , Lee M. Wetzler * 1 Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America, 2 Division of Allergy, Immunology and Rheumatology, ˜ ˜ University of Rochester Medical School, Rochester, New York, United States of America, 3 School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil, 4 Department of Pathology, Tufts University School of Medicine, Cummings School of Veterinary Medicine and Tufts Medical Center, Boston, Massachusetts, United States of America Abstract Background: Francisella tularensis causes severe pulmonary disease, and nasal vaccination could be the ideal measure to effectively prevent it. Nevertheless, the efficacy of this type of vaccine is influenced by the lack of an effective mucosal adjuvant. Methodology/Principal Findings: Mice were immunized via the nasal route with lipopolysaccharide isolated from F. tularensis and neisserial recombinant PorB as an adjuvant candidate. Then, mice were challenged via the same route with the F. tularensis attenuated live vaccine strain (LVS). Mouse survival and analysis of a number of immune