基于高通量测序的冷冻南极磷虾中细菌菌群结构分析-食品安全质量.PDF

7 7 Vol. 7 No. 7 2016 7 Journal of Food Safety and Quality Jul. , 2016 江艳华, 姚 琳, 李风铃, 郭莹莹, 朱文嘉, 王联珠* (, , 266071) 摘 要: 目的 方法 , ; DNA, 结果 , 1% (Bacillus)(Enterococcus)(Cronobacter)(Alkaliphilus) (Sarcina)(Lactococcus)(Paenibacillus)(Sediminibacterium) (unclassified) (Ralstonia) (Sphingomonas) (Phenylobacterium), 85.04% , (Pseudoalteromonas)(Psychrobacter)(Psychromonas), 64.43% 33.47% 1.75% 1.10%结论 , 关键词: ; ; Analysis for bacterial flora structure of frozen Antarctic krill Euphausia superba by high-throughput sequencing technique * JIANG Yan-Hua, YAO Lin, LI Feng-Ling, GUO Ying-Ying, ZHU Wen-Jia, WANG Lian-Zhu (Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture , Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China) ABSTRACT: Objective To analyze the structure of total and cultured bacterial flora in frozen Antarctic krill Euphausia superba. Method Total bacterial flora was analyzed via high-throughput sequencing technique after extracting the metagenomic DNA. Cultured bacterial flora was analyzed via high-throughput sequencing technique