响应面法优选苦豆子多糖活性炭脱色工艺.pdf

响应面法优选苦豆子多糖活性炭脱色工艺Δ 1＊ 1 1 1 2# 单晓菊 ，邸明磊 ，张 岩 ，马丽娜 ，陶遵威 （1.天津中医药大学，天津市 300193；2.天津市医药科学研究 所，天津市 300020） 中图分类号 R284.2；R283 文献标识码 A 文章编号 1001-0408（2011）47-4443-04 摘 要 目的：优选苦豆子多糖活性炭脱色工艺。方法：以活性炭添加量（W/ V）、脱色时间、脱色温度为考察因素，通过单因素试 验（以脱色率、多糖损失率为指标）和响应面法（以脱色率为指标）优化苦豆子多糖活性炭脱色工艺。结果：最佳脱色工艺为活性炭 添加量（W/ V）占0.99％，时间59.6min ，温度40.3℃，其脱色率可达到66.4％。结论：该脱色工艺适合工业化生产。 关键词 苦豆子；多糖；脱色；响应面法 Optimization of Decolorization Technology of Sophora alopecuroides Polysaccharide with Activated Carbon by Response Surface Analysis SHAN Xiao-ju ，DI Ming-lei ，ZHANG Yan ，MA Li-na （Tianjin University of Traditional Chinese Medicine ， Tianjin 300193，China ） TAO Zun-wei （Tianjin Institute of Medical Sciences ，Tianjin 300020，China ） ABSTRACT OBJECTIVE ：To optimize the decolorization technology of Sophora alopecuroides polysaccharide with activated car- bon. METHODS ：The decolorization technology of S. alopecuroides was optimized by the single-factor experiment （decolorization ratio and loss rate of polysaccharide as index ）and response surface method （decolorization ratio as index ），with adding amount of activated carbon addition （W/ V），decolorization time and temperature as factors.RESULTS ：The optimal decolorization technology was as follows ：activated carbon addition （W/ V）accounted for 0.99％，decolorization time 59.6 min ，decolorization temperature 40.3 ℃and the decolrization rate 64.3％. CONCLUSION ：The decoloration technology is applicable for industrialized production. KEY WORDS Sophora alopecuroides ；Polysaccharide ；Decolorization ；Response surface 途径。SEDDS 具有服用方 强度 溶解度，以得到药物溶解度高、自乳化能力强和质量稳定可靠 便，制备简单，适合大规模生 8 的处方。 产等优点，具有极大的发展 由配伍试验可知，HS 15组的自乳化时间显著短于EL 组， 前景和应用价值。