《genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing》.pdf

LETTER doi:10.1038/nature12364 Genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing 1 2 2 3 3 1 1 1 Zhigang Xue *, Kevin Huang *, Chaochao Cai , Lingbo Cai , Chun-yan Jiang , Yun Feng , Zhenshan Liu , Qiao Zeng , 1 1 3 2 2 Liming Cheng , Yi E. Sun , Jia-yin Liu , Steve Horvath Guoping Fan Mammalian pre-implantation development is a complex process Intriguingly, cluster analyses showed that both pronuclear and involving dramatic changes in the transcriptional architecture1–4. zygote (1-cell stage) embryos clustered closely together but away from We report here a comprehensive analysis of transcriptome dynamics oocytes and cleavage embryos, indicating that the 1-cell stage exhibits a from oocyte to morula in both human and mouse embryos, using distinct transcriptome pattern. Comparisons between mature oocytes single-cell RNA sequencing. Based on single-nucleotide variants in and 1-cell embryos identified 149 differentially expressed genes (false human blastomere messenger RNAs and paternal-specific single- discovery rate (FDR) , 5%, .2-fold change), 79 and 70 of which were nucleotide polymorphisms, we identify novel stage-specific mono- up- and downregulated in 1-cell embryos, respectively (Fig. 1c). Inter- allelic expression patterns for a significant portion of polymorphic estingly, approximate