why assembling plant genome sequences is so challenging为什么植物基因组序列组装如此具有挑战性.pdf

Biology 2012, 1, 439-459; doi:10.3390/biology1020439 OPEN ACCESS biology ISSN 2079-7737 /journal/biology Review Why Assembling Plant Genome Sequences Is So Challenging Manuel Gonzalo Claros 1,2,*, Rocío Bautista 2, Darío Guerrero-Fernández 2, Hicham Benzerki 1,2, 1 1 Pedro Seoane and Noé Fernández-Pozo 1 Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Malaga, 29071 Málaga, Spain; E-Mails: bhicham538@ (H.B.); seoanezonjic@uma.es (P.S.); noefp@uma.es (N.F.-P.) 2 Bioinformatics Andalusian Platform, Bio-innovation Building, University of Malaga, 29590 Málaga, Spain; E-Mails: rociobm@uma.es (R.B.); dariogf@uma.es (D.G.-F.) * Author to whom correspondence should be addressed; E-Mail: claros@uma.es; Tel.: +34-951-952-787; Fax: +34-952-132-041. Received: 16 July 2012; in revised form: 5 September 2012 / Accepted: 6 September 2012 / Published: 18 September 2012 Abstract: In spite of the biological and economic importance of plants, relatively few plant species have been sequenced. Only the genome sequence of plants with relatively small genomes, most of them angiosperms, in particular eudicots, has been determined. The arrival of next-generation sequencing technologies has allowed the rapid and efficient development of new genomic resources for non-model or orphan plant species. But the sequencing pace of plants is far from that of