免疫磁珠分选技术及应用.ppt

* To isolate a cell according to two different markers: A positive selection followed by a second positive selection * What is possible for cells, is also possible for molecules. * What is possible for cells, is also possible for molecules. * What is possible for cells, is also possible for molecules. * What is possible for cells, is also possible for molecules. * Cytokine Secretion Assay is a completely new technology (got a different colour on the slide). Analysis and also enrichment of extremely rare antigen-specific T cells, for example. * Cytokine Secretion Assay is a completely new technology (got a different colour on the slide). Analysis and also enrichment of extremely rare antigen-specific T cells, for example. * Antigen-specific restimulation leads to cytokine secretion Catch reagent forms an artificial surface to catch the cytokine which is secreted. Catch reagent is composed of two antibodies, one of them directed against the cytokine. Labeling with detection antibody. Indirect magnetic labeling is optional for enrichment. Without enrichment (no columns): detect frequencies between 0.01-0.1% of total cells. With enrichment: detect frequencies down to 0.0001 % (1 cell in 10e6 cells!!) In addition, cells stay alive during enrichment and detection and can be taken into culture or used for molecular analysis. * Antigen-specific restimulation leads to cytokine secretion Catch reagent forms an artificial surface to catch the cytokine which is secreted. Catch reagent is composed of two antibodies, one of them directed against the cytokine. Labeling with detection antibody. Indirect magnetic labeling is optional for enrichment. Without enrichment (no columns): detect frequencies between 0.01-0.1% of total cells. With enrichment: detect frequencies down to 0.0001 % (1 cell in 10e6 cells!!) In addition, cells stay alive during enrichment and detection and can be taken into culture or used for molecular analysis. * Antigen-specific restimulation leads to cytokin