拟南芥激活标记突变体库的构建及突变体基因的克隆_李志邈.pdf
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, Journal of Plant Physiology and Molecular Biology 2005, 31 (5): 499506 499
1,3 2 1* 2
1 2
( 3 1 0 0 2 9
3 3 1 0 0 2 1 )
2 0 0 0 3 2
:(activation tagging) (quantitative trait
loci, QTL)
(Arabidopsis
thaliana) ( ga i n-of-
pSKI015 function)(activa-
Basta tion tagging)
20 000 (T1 )
38
(1) (enhancer)T-
DNA Southern
T-DNA (plasmid DNA
rescue) TAIL-PCR(Thermal asymmetric interlaced-PCR) (2)
T-DNA T1
(3) TAIL-PCR
: (4)
TAIL-PCR
:Q74 (5)
(Arabidopsis thaliana ) (Kardailsky 1999; Ito
(Crucifereae) Meyerowitz 2000; Borevitz 2000; Huang 2001;
Nakazawa 2001, 2003; Park 2003)
(Petunia hybrida)(Populus)
(Lycopersicon esculentum)
(Zub ko 200 2;
125 Mb
Busov 2003; Mathews 2003)
2000 12 (The Arabidopsis Genome
Initiative 2000) / TAIL-PCR
T-DNA
1
1.1
(lo
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