四川主栽茶树品种的多酚氧化酶活性.pdf

贵州农业科学2011，39(1)：51～53 Sciences GuizhouAgricultural 四川主栽茶树品种的多酚氧化酶活性 陈昌辉，齐桂年，汪艳霞 (四川农业大学园艺学院，四川雅安625014) [摘 要]为了实现控制PPO酶，提高四川红、绿茶加工品质，对四川主要产荼区具代表性的5个茶树 栽培品种鲜叶的多酚氧化酶(PPO)进行粗提，测定并比较不同品种的酶活特性。结果表明，当底物邻苯二酚 的量为0．3～1．2mI。时，各个品种的酶活力随浓度增加而升高，而超过1．2mL时，酶活力下降；在25～45℃ 种的酶活力丧失程度不同；各品种的酶活力的适宜pH相对偏碱；相同浓度的3种抑制剂对各品种的酶活力 均有一定的抑制效果。 [关键词]茶树；鲜叶；多酚氧化酶 [中图分类号]$571．1 [文献标识码]A Activitiesof on Oxidase TeaCultivarsGrowninSichuan StudyPolyphenol CHEN Gui—nian，WANGYan-xia Chang—hui，QI Horticulture，Sichuan 625014。China) (Collegeof AgriculturalUniversity。Ya’an，Sichuan obtainedfrom5 teacuhivars in Abstract：Crudeoxidase(PPO)was polyphenol separately grown Sichuanand wereexaminedtoenhance of teaandblacktea enzymaticproperties processingqualitiesgreen inSichuan．TheresultsshowedthatPP0activitiesincreasedwiththeincreaseofcatecholsubstrate grown volumewhenthevolumeofcatecholsubstratein0．3～1．2mL，anddecreasedwheneatechol ranged substratevolumewas than1．2mL；PPOactivitiesalsoincreasedwiththeincreaseof greater temperature whenthe in25～45℃，anddecreasedwhenthe was than45℃； temperatureranged temperaturegreater ThePPOactivitiesof5teacultivarsdecreasedin undertheconditionof50～90℃hotwater varyingdegree treatmentfor20 couldactunderalkaline thefiveinhibitorsshowedsome minutes；PPO conditions；All ofinhibitiontoPPOactivities． degree tea