鸭梨果肉多酚氧化酶的活性研究与纯化-河北农业大学学报.pdf

33 5 Vol.33 No.5 20 1 0 9 J OURNA L OF AGRICULT URAL UNIVERSIT Y OF H EBEI 　 　 Sep .2 0 1 0 :1000-1573(2010)05-0045-05 1, 2 1 1 2 李桂琴 , 　刘　坤, 　高志华, 　张玉星 ① (1. , 050061;2. , 071001) :(Py rus bretschneid er i Re d.)。 , , pH 、、、、 PPO ;30%80%、Sep adex G200 HiPrepTM 16/10 QXL , SDS。: PPO pH 6.4; 25 ℃;20 ～25 ℃;; 34 mmol/LNaCl ;, 。, PPO, 43 kD。PPO PPO 。 　　:;(PPO);; :S 661.2 :A Study on activity and purification of polyphenol oxidase from pulp in Yali pear 1, 2 1 1 2 LI Gui-qin , LIU Kun , GAO Zhi-hua , ZHANG Yu-xing (1.College of Bioscience and Engineering, Hebei University of Economic and Business, S ijiaz uang 050061, C ina; 2.College of H orticulture, Agricultural University of H ebei, Baoding 071001, C ina) Abstract:T e polyp enol oxidase (PPO)from pulp in Yali pear(P y rus br etschn eid er i Re d.) w as extracted and purified, and its activity w as studied.Effects of t e factors suc as pH val- ue, temperature, t ermostability, in ibitor, different mature periods and different parts of pulp etc.on PPO activity w ere investigated wit t e pulp in Yali pear as materials.By using catec ol as t e substrate, t e PPO activity w as identified using t e product of enzymatic reac- tion.T e crude preparation of t e PPO w as purified from pulp of Yali pear by successive steps of ammonium sulfate fractionation wit saturation 30%, ammonium sulfate fractionation w it