temporal differential proteomes of clostridium difficile in the pig ileal-ligated loop model梭状芽胞杆菌的时序差异蛋白质组在猪ileal-ligated循环模型.pdf

Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model 1,2. 1. 3 4 5 Tavan Janvilisri , Joy Scaria , Ching-Hao Teng , Sean P. McDonough , Robin D. Gleed , 5 6 1 1 Susan L. Fubini , Sheng Zhang , Bruce Akey , Yung-Fu Chang * 1 Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America, 2 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand, 3 Institute of Molecular Medicine, National Cheng Kung University Medical College, Tainan, Taiwan, 4 Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America, 5 Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America, 6 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York, United States of America Abstract The impact of Clostridium difficile infection (CDI) on healthcare is becoming increasingly recognized as it represents a major cause of nosocomial diarrhea. A rising number of CDI cases and outbreaks have been reported worldwide. Here, we developed the pig ileal-ligated loop model for semi-quantitative analysis comparing temporal differential proteomes in C. difficile following in vivo incubation with in vitro growth using isobaric tags for relative and absolute quantification (iTRAQ). Proteins retrieved from the in vitro cultures and the loop contents after 4, 8, and 12 h in vivo incubation were subjected to in-solution digestio