susceptible and protective hla class 1 alleles against dengue fever and dengue hemorrhagic fever patients in a malaysian population敏感和保护hla类1等位基因对登革热和登革出血热病人在马来西亚的人口.pdf

Susceptible and Protective HLA Class 1 Alleles against Dengue Fever and Dengue Hemorrhagic Fever Patients in a Malaysian Population 1 2 3 1 Ramapraba Appanna , Sasheela Ponnampalavanar , Lucy Lum Chai See , Shamala Devi Sekaran * 1 Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia, 2 Department of Infectious Diseases, University Malaya Medical Centre, Kuala Lumpur, Malaysia, 3 Department of Pediatrics, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia Abstract Background: The human leukocyte antigen alleles have been implicated as probable genetic markers in predicting the susceptibility and/or protection to severe manifestations of dengue virus (DENV) infection. In this present study, we aimed to investigate for the first time, the genotype variants of HLA Class 1(-A and -B) of DENV infected patients against healthy individuals in Malaysia. Methodology/Principal Findings: This study was carried out with 92 dengue disease patients and 95 healthy controls from three different ethnic groups (Malay, Chinese and Indian) in Malaysia. All patients with clinical and laboratory confirmation of DENV infection were typed for the HLA-A and B loci, using polymerase chain reaction-sequence specific primer techniques. In our total population, a significant increase for HLA-B*53 (P = 0.042, Pc = 1.008) allele and a significant decrease for A*03 (P = 0.015, Pc = 0.18, OR = 5.23, 95% CI = 1.19–23.02) and B*18 (P = 0.017, Pc = 0.408) alleles were noted in DHF patients as compared to healthy donors. We also observed that in the Malay DHF patients, allele B*13 (P = 0.049, Pc = 1.176, OR = 0.18, 95% CI = 0.03–0.90) was present at a