aurintricarboxylic acid is a potent inhibitor of influenza a and b virus neuraminidasesaurintricarboxylic酸是一个强有力的甲型和乙型流感病毒的神经氨酸苷酶抑制剂.pdf

Aurintricarboxylic Acid Is a Potent Inhibitor of Influenza A and B Virus Neuraminidases 1,3. 1. 1 3 2 Anwar M. Hashem , Anathea S. Flaman , Aaron Farnsworth , Earl G. Brown , Gary Van Domselaar , Runtao He2, Xuguang Li1,3* 1 Centre for Biologics Research, Biologics and Genetic Therapies Directorate, HPFB, Health Canada, Ottawa, Ontario, Canada, 2 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, 3 Department of Biochemistry, Microbiology and Immunology, and Emerging Pathogens Research Centre, University of Ottawa, Ottawa, Ontario, Canada Abstract Background: Influenza viruses cause serious infections that can be prevented or treated using vaccines or antiviral agents, respectively. While vaccines are effective, they have a number of limitations, and influenza strains resistant to currently available anti-influenza drugs are increasingly isolated. This necessitates the exploration of novel anti-influenza therapies. Methodology/Principal Findings: We investigated the potential of aurintricarboxylic acid (ATA), a potent inhibitor of nucleic acid processing enzymes, to protect Madin-Darby canine kidney cells from influenza infection. We found, by neutral red assay, that ATA was protective, and by RT-PCR and ELISA, respectively, confirmed that ATA reduced viral replication and release. Furthermore, while pre-treating cells with ATA failed to inhibit viral replication, pre-incubation of virus with ATA effectively reduced viral titers, suggesting that ATA may elicit its inhibitory effects by directly interacting with the virus. Electron microscopy revealed that ATA induced viral aggregation at the cell surface, p