SteadyState ProbePartitioning FRET A Simple and Robust Tool for the Study of Membrane Pha.pdf

1 SteadyStateProbePartitioningFRET:ASimpleand RobustToolfortheStudyofMembranePhaseBehavior 1 JeffreyT.Buboltz DepartmentofPhysicsandAstronomy,ColgateUniversity,Hamilton,NewYork,13346 Abstract—An experimental strategy has been developed Coexisting Coexisting Single Single Coexisting Coexisting specifically for the study of composition-dependent phase phasesphases phasephase phasesphases behavior in multi-component artificial membranes. The strategy is based on steady-state measurements of fluorescence resonance energy transfer between freely diffusing membrane probe populations, and it is well suited for the rapid generation of large data sets. Presented in this paper are the basic principles that guide the experiment’s design, the derivation of an underlying mathematical model that serves to interpret the data, and experimental results that confirm the model’s predictive power. I. INTRODUCTION TT TTT TT EE