乌头与贝母配伍化学成分变化的UPLCQ-TOFMS.pdf

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第16 期, 1920～1928 ACTA CHIMICA SINICA No. 16, 1920～1928 ·研究论文· 乌头与贝母配伍化学成分变化的 UPLC/Q-TOFMS 研究 王 超a,b 王宇光*,b 梁乾德b 让蔚清a 肖成荣b 高 月*,b (a 南华大学生命科学研究中心环境医学与放射卫生研究所 衡阳 421001) (b 军事医学科学院放射与辐射医学研究所药理与毒理研究室 北京 100850) 摘要 利用UPLC/Q-TOFMS 对中药十八反药对——乌头贝母配伍的合煎液与合并液进行检测, 经过MassLynx 4.1 分 析其化学成分存在差异, 发现合煎后次乌头碱水解受到部分抑制, 而其他主要的双酯型二萜生物碱溶出较少且水解较 彻底, 乌头与川贝配伍合煎的毒性相对较小, 因而可能通过合理炮制减毒后应用于临床治疗. 关键词 十八反; UPLC; Q-TOFMS; 液质联用; 乌头; 贝母 Analysis of Chemical Composition in Combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with Multivariate Statistical Analysis Wang, Chaoa,b Wang, Yuguang*,b Liang, Qiandeb Rang, Weiqinga Xiao, Chengrongb Gao, Yue*,b a ( Institute of Environmental Medicine and Radiation Hygiene, Research Center of Life Science, University of South China, Hengyang 421001) (b Institute of Radiation and Irradiation Medicine, Academy of Military Medical Sciences , Beijing 100850) Abstract An ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOFMS) based chemical profiling approach to evaluate chemical constitution between mixed decoction and co-decoction of Radix aconiti-Fritillaria combination of the Eighteen Incompatible Medications (Shi Ba Fan) was proposed. Two different kinds of decoctions, namely Radix aconiti-Fritillaria co-decoction: water ext