达的密码子偏性-华南农业大学学报.pdf

第 31 卷第1 期 华南农业大学学报 Vol. 31 , No.l Joumal of South China Agricultural University Jan. 2010 2010 年 1 月 鸡 Z 染色体基因 达的密码子偏性 饶友生l ，梁菲菲2 ，王棒凤l ，柴学文l ，张细权2 (1江西教育学院生物技术研究所，江西南昌 330029;2 华南农业大学动物科学学院，广东广州 510642) 摘要:从 NCBI 数据库(http: //酬w. ncbi. nlm. nih. gov/project的napview/map) 下载鸡 Z 染色体上全部基因完整的 cDNA，最终共有 626 个基因的 CDS 序列纳入统计分析.使用 CodonW ( 1. 4. 2) 进行密码子偏性分析，确定了 CGG、 AGC、 UGC 等26 个密码子为Z 染色体基因表达的最优密码子.对应分析表明，影响鸡 Z 染色体基因表达的密码 子偏性的主要因素分别为 GC3s、基因的表达丰度、GC 含量、CDS 长度及氨基酸的疏水性.鸡 Z 染色体基因表达的 密码子用法受到了核昔酸组成偏好的显著影响，这种核昔酸组成偏好很可能是突变偏畸、固定偏畸及基因转换导致 的.对于鸡这种群体有效规模较大的群体，密码子的偏性更有可能是核昔酸组成偏好及选择等因素综合作用的结果. 关键词:鸡;z 染色体;密码子;偏性 中图分类号:Q953.3 文献标iR码:A 文章编号:lool-411X( 2010 )01-0070-05 Study on the Codon Bias of Genes Expression in GGAZ 1 2 1 1 RAO You-sheng , LIANG Fei-fei , WANG Zhang-feng , CHAI Xue-wen , ZHANG Xi叩IaI12 (1 Department of Biological Technology ,Jiangxi Institute of Education ,Nanchang 330029 , China; 2 College of Animal Science , South China Agricultural University , Guangzhou 510642 ,China) Abstract: The total cDNA sequences were downloaded from NCBI dateset ,and 626 CDS sequences were finally included in analyses. By the use of CodonW ( 1. 4. 2) , 26 codons (eg. CGG , AGC , UGC et al. ) were identified as optimal codons. The corresponding analyses indicted that the main factors inf1uencing codon usage of genes in GGAZ were GC3 s , expression level , GC content , CDS length as well as Hydropho- bicity of amino acids. As the codon usage of genes in GGAZ was mostly inf1ucenced by nucleotide compo- sition , the bias of nucleotide composition