高效液相色谱法测定首乌丸中大黄素的含量.pdf

118 Chinese Journal of Biochemical Pharmaceutics 2009 30 2 1, 2 2 2 2 李 静 , 张 莉 , 曹 玲 , 王 玉 ( 1. 中国药科大学, 江苏 南京 210009; 2. 江苏省食品药品检验所, 江苏 南京, 210008) : 建立首乌丸中大黄素含量的测定方法 运用正交实验优 样品处理的提取条件, 采用高 效液相色谱法检测大黄素含量, 色谱柱为 UltimateXB-C ( 5 Lm, 250 mm @ 4. 6 mm) , 流动相为甲醇-0. 1% 磷酸溶液 18 ( 87B13) , 检测波长254 nm 大黄素在 1. 89~ 60. 37 Lg 范围内与峰面积呈良好的线性关系, r 0.999 8, 游离大 黄素和总大黄素的平均加样回收率分别为100. 16% 和 99. 87% , RSD 分别为0. 91% 和1.50% 此法简便准 确快捷, 可用于首乌丸的质量控制 : 首乌丸; 大黄素; 高效液相色谱法 : R927.7 :A : 1005-1678(2009) 02-0118-03 Determination of the content of Emodin in Shouwu Pills by HPLC 1,2 2 2 2 LI Jing , ZHANG Li , CAO Ling , WANG Yu (1. China Pharmaceutical University, Nanj ing 210009, China; 2. Jiangsu Institutef or ood Drug Control, Nanj ing 210008, China) Abstract: Purpose To establish a HPLC method for the determination of Shouwu Pills. Methods After using the orthogonal test to optimize extracting conditions, Emodinwas separated on UltimateXB-C ( 5 Lm, 250 18 mm @ 4. 6 mm) column and detected at 254 nm, with methanol-0. 1% phosphoric acid solution( 87B13) as a mobile phase. Results There was good linearity from 1. 89-60. 37 Lg, r 0. 999 8, in separation of Emodin. The average recovery of free emodin and total emodin separately was 100. 16% with RSD of 0. 91% and 99. 87% with RSD 1. 50% . Conclusion This method is accurate, simple and rapid. This study provides the reference to establish the quality standard for Shouwu Pills. Key words: Shouwu Pill; Emodin; HPLC