增加罐压对重组大肠杆菌高密度发酵的影响.pdf

Biotechnology and Bioprocess Engineering 16: 488-494 (2011) DOI 10.1007/s12257-010-0301-y RESEARCH PAPER Characteristics of High-cell Density Cultivation of Recombinant Escherichia coli Producing RHLC Using the Fermentor Pressure Shifting Strategy Lei Chi, Dai-Di Fan, Xiao-Xuan Ma, Yan-E Luo, and Chen-Hui Zhu Received: 1 September 2010 / Revised: 20 December 2010 / Accepted: 3 January 2011 © The Korean Society for Biotechnology and Bioengineering and Springer 2011 Abstract To increase the biomass and production of re- Keywords: recombinant human-like collagen, oxygen combinant human-like collagen (RHLC), the effect of con- transfer capacity, recombinant Escherichia coli, fermentor trolled fermentor pressure during fed-batch cultivation was pressure, kinetic model investigated using recombinant Escherichia coli producing RHLC. This study focused primarily on the effects of the fermentor pressure on the oxygen transfer capacity. A two- 1. Introduction step exponential feeding strategy was used to control the specific growth rate at 0.2 and 0.1/h in the fed-batch and Recombinant human-like collagen (RHLC) is a novel bio- induction phase, respectively. A kinetic model of cell growth material produced by recombinant E. coli. RHLC retains not was developed, and the specific growth rate, specific glucose only the original functions of collagen, but also has a unique uptake rate, concentration of extracellular DNA, and per- chemical structure and functions that are superior to those of centage of plasmid loss were calculated and detected. The animal collagen [1]. Thus far, few studies have examined the results demonstrated that increasing the fermentor pressure applications of RHLC in ti