RP-HPLC测定木通药材中皂苷类成分的含量.pdf

RP-IHPLC 测定木通药材中皂普类成分的含量 高慧敏，王智民* ，付雪涛，王维皓，王冠( 中国中医利学院中药研究所，北京100700) 摘要: 目的 建立木通药材中2 种皂普类成分同时用HPLC测定的方法。方法 采用Phenomenex Luna C,$(4.6 mmx250 mm,5 pm) 柱，以甲醇一乙睛一水一磷酸(20:20:60:0. 1) 为流动相，流速1.0 mL - min- ，检测波长为203 rim，柱温35 `C 。结果 saponin 马，和mutong- saponin C 分别在0.25 - 4.0 pg,0.21- 3.4 pg 内呈良好的线性关系，平均加样回收率分别为98. 11%和101. 19% 。对3 个基源( 三叶、 五叶、白木通) 的木通药材共30 个样品进行了含量测定，并比较了两者在原植物根、茎、叶、果实( 共 10 个样品) 中的分布。结论 白 木通和三叶木通中均含有2 种被测成分，五叶木通大部分样品未检出。 关键词:木通;皂普Pn ;木通皂普C;高效液相色谱法 中图分类号二8284 文献标识码:A 文章编号:1001- 2494(2006)01一〕20 - 04 Determination of 1r iterpenoid Saponins in Cauf s akebfae by GAO Hui-min ,WANG Zhi-min . ,FU Xue-tao,WANG Wei-hao ,WANG Guan (Institute of Chinese Materia Medica , China Acade- my of Chinese Medical Sciences , Beji ing 10(Y700 ,China ) ABSTRACT ;OBJECTIVE To establish a HPLC method for the determination of saponin 马, and mutongsaponin C in Caulis akebi- ae. ME THODS The samples were separated at 35 ℃ using a Phenomenex Luna C,8 column with methanol-acetonitrile-water-phosphoric acid (20:20:60:0. 1) as mobile phase. Flowrate was at 1.0 mL ·min 一’and the detection wavelength was at 203 ， RESULTS The cali- bration curves of saponin P and mutongsaponin C were linear over the ranges of 0.25 一4.0 pg, 0.21一3.4 pg and the average recoveries were 98. 1% and 101. 2% ，respectively. Forty crude materials rf om the root ,stem ,leaf and fur it , collected fro m diff erent areas , were deter- mined. CONCLUSION Saponin P, and mutongsaponin C can be regarded as