决明子有效成分黄酮类化合物的提取研究 中药学与药物化学专业毕业设计 毕业论文.doc

摘 要 决明子是一种常用中药，富含黄酮，蒽醌和多糖，具有广泛的药理活性。现代药理学研究又表明，决明子具有降血压、降血脂，保肝及抑菌等活性，被应用于多种降脂减肥的成方制剂中。同时，决明子又可作食用中药，是保健饮料的良好原料。实验测定了决明子中黄酮类化合物的含量，含量测定应用紫外—可见分光光度法进行测定，并使用NaNO2-Al(NO3)3-NaOH显色体系75％乙醇为提取剂，80℃下提取，提取时间3.5h，料液比1:30时C=96.838A-2.8675，相关系数R2=.9951。又测得提取液吸光度为0.428，黄酮浓度为37.0μm/mL，提取率为88.61%。关键词：明子，黄酮类化合物，乙醇，提取工艺 Abstract Semen Cassiaen a traditional Chinese herbal medicine, which is a rich source of flavones,polysaccharides and anthraquinone,having a widespread activity in biology. Modern pharmacology study also shows that, cassia seed also have fall blood pressure, fall hematic fat, protect liver and bacteriostasis activities, be applied to various cholesterol-lowering diet drugs in preparations. Meanwhile, cassia seed and eating Chinese traditional medicine, is the good health drink raw material.Some chemical separation factors were adopted to extract flavonoids. This experiment determined the content of flavonoids in cassia seed, and cassia seed the extraction technology of flavonoids were studied.Ethanol Condensing Reflux Extraction were applied to extract flavonoids．When extracting，Five extraction factors of flavonoids from Semen Cassiaen were investigated and compared, including extracting time ，ethanol concentration，extracting temperature and ratio of liquid to solid. Again the optimization of process conditions for orthogonal experiment. Quantification was performed by means of UV–Vis spectrophotometry with chromogenic system of NaNO2–Al (NO3)3–NaOH. Experiments show that that the ethanol extraction was better than the other．The found optimum extraction conditions were that the ethanol concentration was 75% (v/v), solid–liquid ratio was 1:30, extracting temperature was 80°C, extracting time was3.5h. Using the concentration of rutin standard solution as abscissa and the absorbency as y-coordinate, the linear chart was constructed and the standard curve the regression equation was C=96.838A-2.8675(R2=0.9951). And measurement extract spectrophotometry for 0.428 and the