CD4_CD25_调节性T细胞抑制小鼠自身免疫性甲状腺炎的发生.pdf

# 102 # 2005 21 + + ¹ CD4 CD25 T º » ( 江苏大学医学技术学院免疫学研究室, 镇江212001) R392 A 1000-484X(2005)02-0102-04 + + [ ] : CD4 CD25 Treg ( EAT) : + + + + + - ( MACS) CD4 CD25 T , IFN-C CD4 CD25 T CD4 CD25 T + + + + , CD4 CD25 T EAT : MACS CD4 CD25 T + + 85% ~ 94% , Fo P3 , T IFN-C ; CD4 CD25 T - + + CD25 T , T EAT : CD4 CD25 Treg T + + [ ] CD4 CD25 T ; ; ; + + Study of CD4 CD25 regulatory T cell in preventing adoptive tran fer of experi- mental autoimmune thyroiditi WANG Sheng-Jun , X UHua-Xi, WANG Yong-Zhong, H UJia-Bo, MA Bin. Department of Immunology , Jiangsu Uni versity School of Medical Technology , Zhenjiang 212001, China + + + [Ab tract] Objective: To investigate the immunosuppression of mouse CD4 CD25 regulatory T (Treg) cell. Method :Mouse CD4 + CD25 T cells were isolated by MACS ( magnetic cell sorting) and identified by FCM( flow cytometry) . Their suppression on the proliferation + - and the IFN-C production of CD4 CD25 T effector cell were analyzed by cell proliferation assay and ELISA, re