蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖.pdf

280 2008, Vol. 29, No. 06 食品科学 ※分析检测 张书芬，史萍萍，王全林，沈 坚，傅 晓 (宁波市产品质量监督检验所，浙江宁波 315041) ：本实验对反相高效液相色谱示差折光检测法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖的方法进行了详 细地探讨，结果表明：均匀称取约2.5g 样品，加30ml 水溶解后，再用乙腈定容至50ml ，既能够有效地提取蜂蜜 中的糖类物质，又保证了和流动相的相对一致性。同时对样品溶解时的分层机理作了初步推测。用该方法处理所 得的样品稳定性高，测试结果重复性好。该法加标回收率均在97%～103% 之间，相对标准偏差均在0.8%～1.6% 之间。采用本研究方法测试了8 种不同蜂蜜样品，均获得满意结果。 ：液相色谱示差折光法；蜂蜜；果糖；葡萄糖；蔗糖；麦芽糖 Determination of Fructose, Glucose, Sucrose and Maltose in Honey by Liquid Chromatography Refractive Index Detector ZHANG Shu-fen ，SHI Ping-ping，WANG Quan-lin ，SHEN Jian，FU Xiao (Ningbo Supervision and Inspection Institute of Product Quality,Ningbo 315041, China) Abstract ：This study discussed the method for determination of fructose, glucose, sucrose and maltose content in honey by reverse-phase high performance liquid chromatogram refractive index detector (HPLC-RID). Results indicated that not only the carbohydrate can be extracted from the honey effectively, but also the mobile phase relative coherence can be guaranteed by dissolving sample (2.5 g) with distilled water (30 ml) and then diluting to 50 ml with acetonitrile. Soluble mechanism was also presumed. The excellent stability and repeatability are obtained by the developed pretreatment method. The spiked recoveris is in the range of 97%～103% with relative standard deviation of 0.8%～1.6%. Eight different honey samples were detected and good results are obtained. Key words ：HPLC-RID ；honey ；ructose ；glucose ；sucrose ；maltose 中图分类号：TS207.7 文献标识码：A 文章编号：1002-6630(2008)06-0280-04 蜂蜜是具有悠久历史的经典的保健品之一，纯正 外， 离子色谱脉冲安培法[5-6] 、液相色谱蒸发光散射检 的、质量好的蜂蜜具有很高的营养价值和功效，所以 测法(HPLC-ELSD)[7]