响应面试验优化超声波辅助提取莲房原花青素工艺.pdf

40 2016, Vol.37, No.12 食品科学 ※工艺技术 响应面试验优化超声波辅助提取 莲房原花青素工艺 李佳桥，余修亮，曾林晖，邓泽元，李红艳* （南昌大学食品科学与技术国家重点实验室，江西 南昌 330047 ） 摘  要：在单因素试验的基础上，采用响应面试验研究乙醇体积分数、液料比、超声波功率和超声时间对莲房原花 青素得率的影响，通过建立超声波辅助提取莲房原花青素的多元回归模型，优化莲房原花青素的提取工艺参数。结 果表明，乙醇体积分数对莲房原花青素得率的影响最大，其次是液料比和超声波功率，超声时间对得率的影响相对 较小。在乙醇体积分数45%、液料比21 ∶1 （mL/g ）、超声波功率700 W 、超声时间15 min时，莲房原花青素得率最 大，为6.81%，与模型理论预测值相近，说明该模型回归性良好，试验的拟合程度高，可以用于莲房原花青素得率 的预测，为莲房原花青素作为天然抗氧化剂的应用提供一定的科学数据。 关键词：莲房；原花青素；提取工艺；超声波；响应面 Response Surface Methodology for Optimization of Ultrasonic-Assisted Extraction of Proanthocyanidins from Lotus Seed Pot * LI Jiaqiao, YU Xiuliang, ZENG Linhui, DENG Zeyuan, LI Hongyan (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract: One-factor-at-a-time method (OFAT) and response surface methodology (RSM) were adopted to study the effects of ethanol concentration, liquid/solid ratio, ultrasonic power and ultrasonic treatment time on the extraction yield of proanthocyanidin from lotus seed pot (LSP). A multiple regression model was established to optimize the parameters for ultrasonic-assisted extraction of LSP. The results indicated that ethanol concentration had the most significant impact on the extraction yield of LSP, followed by liquid/solid ratio and ultrasonic power, while the impact of ultrasonic treatment time was relatively low. When the extraction was carried out using 45% ethanol as the extractant at a liquid/solid ratio of 21:1 (mL/g) with ultrasonic treatment for 15 min at 700 W, the maximum yield of LSP of 6.81% was obtained, which was close to the model-predict