何首乌及其常见混淆品的matK基因序列分析及鉴别_生书晶.pdf

DO I :10.13863/j .issn1001 -4454.2010.11.029 ournalofChineseMedicinalMaterials　　 33 11 2010 11 ·1707 · Rwandanmedicinalplantextractsfortheirantmiicrobial andantiviralactivities[ ]..Ethnopharmacology, 2002, 79 [1] .[M].: (2 ):155-163. , 200 6:3563-3564. [4] , , , . [2] OlajideOA, AladaAR.Studiesontheanti-inflammatory S180 [ ]., 2006, 9 (5):397 - propertiesofEntadaabyssinica[ ] .Fitoterapia, 2001, 7 2 399. (5):492-496. [5] , , , . [3] CosP, HermansN, DeBruyneT, eta.lFurtherevaluationof [ ]., 2005, 20 (6):487-489. · · 何首乌及其常见混淆品的 matK基因序列分析及鉴别 1, 2 1 1 1 ＊ 生书晶 , 严　萍,郑传进 ,赵树进 (1.中国人民解放军广州军区广州总医院, 广东 广州 510010;2.华南理工大学生物科学与工程学院, 广东 广州5 10641) 　　　: matK, 。: CTAB DNA, matK PCR, TA。: , matK, , ;, ; matK 3。: matK, , matK。 　;matK;; :282.5　　:A　　:1001-4454(2010 )11-1707 -05 AnalysisandAuthenticationofFallopiamultifloraandItsAdulterantsbymatKSequence 1, 2 1 1 1 SHENGShu-jing, YANPing, ZHENGChuan-jin, ZHAOShu-jin (1.DeparmtentofPharmacy, GeneralHospitalofGuangzhouMilitaryCommand, Guangzhou510010, China;2.SchoolofBiological ScienceandEngineeringSouthChinaUniversity, Guangzhou51064 1, China) Abstract　Objective:ToidentifyFallopiamultiflorafromitsadulterantsbycomparingtheirmatKsequences.Methods:Genomic DNAofdifferentmaterialswasextractedusingmodifiedcetyltrimethylammoniumbromide(CTAB)method.Thedouble-strandmatK geneswereamplifiedusingPCRmethodandthensequenced.ThedatawereanalyzedinClustralW andMEGA4.0 softwarepack