20_S_人参皂苷Rg3对血管内皮细胞增殖和迁移的抑制作用.pdf

·1352 · 20 10 37 12 DOI:10.3 971/j .issn.100 0-857 8.2 010.12 .0 05 20 (S )-Rg3 1 2 3 1 辛　颖,姜　新 , 崔俊生 ,倪劲松 Inhibitory Effect of 20(s)-Ginsenoside Rg3 on Proliferation and Migration of Vascular Endothelial Cells 1 2 3 1 XIN Ying ,J IA NG Xin , CU I Jun-sheng , N I J in-song 1.Key Laboratory of Pathobiology , Ministry of Education, J ilin University , Changchun 130021, Chin;2 .Department of Radiotherapy , Cancer Center, The irst Hosp ital of J ilin University; 3 .Dep artment of Pathology , The Third hosp ital of J ilin University Corresp onding Author:NI J in-song, E-mail:nij s@j lu.edu.cn Abstract:Objecti e　T o study the mechanism o inhibitory e ect o ginseno side SPG-Rg3 o n ang io gen- esis.Methods　T o observ e the e ect o SPG-Rg 3 o n the pro li e ratio n and mig ratio n o human umbili- cal v ein endo the lial cells induced by conditioned medium o B 16 melano ma cells (BM CM )by M T T as- say, PCN A immuno luo rescence staining and mig ra tion a ssay.T he V EG F and M M P-9 ex pression o B16 melano ma cells trea ted w ith S PG-Rg 3 w e re analy zed by immuno cy tochemical staining.Results　 T he pr omo ting e ect o BM CM o n pro li er atio n and mig ra tion o vascular endo thelia l cells w as re- duced in SPG-Rg3 g ro up s.M o reo ver, 5μg/ml SPG-Rg3 inhibited the V EG F and M M P-9 ex pressio n o B16 melanoma cells.Conclusion　SPG-Rg 3 can suppress the pro moting e ect o B 16 mela noma cells on pro li eratio n and mig ration o va scular endo thelial cells by decrea sing secretion o V EG F and M M P- 9, thu s indirectly inhibited tumo r angiog enesis. Key words:G inseno side Rg 3;A ng io genesis;U mbilical vein endo thelial cells 　:　探讨2 0(S)-人参皂苷 Rg3 (SPG-Rg 3)抗肿瘤诱导新生血管生成的作用机制。　 采用M T T 法、P CNA 免疫荧光染色和 Boy de n 小室迁移实验, 观察SPG-Rg 3 对B1