常用缓冲溶液的配制方法.pdf

常用缓冲溶液的配制方法 1. 磷酸氢二钠–柠檬酸缓冲液··········································································2 2 ．柠檬酸–柠檬酸钠缓冲液（0.1 mol/L ）··························································2 3 ．甘氨酸–盐酸缓冲液（0.05 mol/L ）·······························································3 4 ．邻苯二甲酸–盐酸缓冲液（0.05 mol/L ）·························································3 5 ．乙酸–乙酸钠缓冲液（0.2 mol/L ）································································3 6 ．磷酸盐缓冲液·························································································3 7 ．磷酸二氢钾–氢氧化钠缓冲液（0.05M ）························································4 8 ．巴比妥钠-盐酸缓冲液（18℃ ）····································································4 9 ．硼酸–硼砂缓冲液（0.2M 硼酸根） ·······························································5 10 ．甘氨酸–氢氧化钠缓冲液（0.05M ）·····························································5 11 ．碳酸钠-碳酸氢钠缓冲液（0.1M ）·······························································5 12 ．Tris–盐酸缓冲液（0.05M ，25℃ ）······························································6 13 ．硼砂-氢氧化钠缓冲液（0.05M 硼酸根）·······················································6 14 ．PBS 缓冲液···························································································6 15. B-R 缓冲溶液配制 ···················································································7 16. 常用pH 缓冲溶液的配制和pH 值································································7 1 1. 磷酸氢二钠–柠檬酸缓冲液 0.2mol/L 0.1mol/L 柠檬酸 0.2mol/L 0.1mol/L 柠檬酸 pH pH Na2HPO4(mL) (mL) Na2HPO4 (mL) (mL) 2.2 0.40 19.