Chapter+11+方法开发课件.ppt

ACQUITY TQD Operation and Maintenance with MassLynx Software Rev. 01110 Appendix A : Page * of 59 The calculation shows the conversion for only one line in the gradient program. The ratio of the column dimensions (20/150=0.133) is applied to all segments to scale the program appropriately * 用相同的等梯度条件例如甲醇：pH7磷酸盐缓冲体系，在不同的反相色谱柱上，测试一对化合物：中性的二氢苊和碱性的阿米替林。把二氢苊的保留因子（保留因子=峰保留时间/空白溶剂峰时间）取自然对数表达为横坐标值，把阿米替林对二氢苊的选择因子（阿米替林与二氢苊的保留因子的比值）表达为纵坐标。 在坐标图中越靠右边的固定相，其反相保留能力越高。在坐标图中越靠上方的固定相，在该流动相条件（pH7)下对阿米替林的极性选择保留越强。 Rev. 05409 Appendix: B Pg. * of 24 Developing Separations for LC/MS Rev. 05409 Appendix: B Pg. * of 24 Developing Separations for LC/MS HPLC到UPLC液相方法转化计算器 广泛的选择性覆盖反相UPLC柱固定相在pH7条件下 (ln [k] acenaphthene) YMC-Pack? ODS-A? YMC Jsphere? ODS–H80 ACQUITY UPLC? BEH C18 Hydrophobicity 062010 SunFire ? C18 YMC-Pack? PolymerC18? Hypersil? CPS Cyano YMC-Pack? CN Waters Spherisorb? S5 P ACQUITY UPLC CSH? Fluoro-Phenyl Nova-Pak? Phenyl YMC-Pack? Phenyl Hypersil? Phenyl Inertsil? Ph-3 YMC-Pack? Pro C4? YMCbasic? Symmetry? C8 YMC-Pack? Pro C8? Nova-Pak? C8 XTerra? MS C18 Symmetry? C18 YMC-Pack? Pro C18? Inertsil? ODS-3 Nova-Pak? C18 YMC Jsphere? ODS–L80 Nucleosil? C18 Waters Spherisorb? ODS2 Waters Spherisorb? ODS1 Resolve? C18 μBondapak? C18 YMC-Pack? ODS–AQ? YMC Jsphere? ODS–M80 Waters Spherisorb? S5CN Nova-Pak? CN HP SymmetryShield? RP8 SymmetryShield? RP18 XTerra? RP8 XTerra? RP18 -0.6 -0.3 0 0.3 0.6 0.9 1.2 1.5 1.8 2.1 2.4 2.7 3 3.3 3.6 -1.5 -0.5 0.5 1.5 2.5 3.5 (ln [a] amitriptyline/acenaphthene) XTerra? MS C8 Luna ? C18 (2) XTerra ? Phenyl Luna ? Phenyl Hexyl ChromolithTM RP-18 Atlantis? dC18 Zorbax? XDB C18 ACT Ace? C18 Zorbax? SB C18 SunFire ? C8 Luna? C8 (2) ACQUITY UPLC? BEHShield RP18 ACQUITY UPLC? BEH C8 ACQUITY UPLC? BEH Phenyl Atlantis? T3 ACQUITY UPLC? HSS T3 ACQUITY UPLC? HSS C18 ACQUITY UPLC? HSS C18 SB Different Reversed Phase UPLC? Column Chemistries Wide Range of Selectivities and Hydrophobicities Selectivity ACQUITY UPLC CSH? ACQUITY