《酶工程》第8章 酶分子定向进化-教学课件（非AI生成）.ppt

**Figure1.?M13phageasusedinphage-displayedlibrariesandastemplatesfornanoscalematerials.?(a)SchematicofM13phagewithphageDNAmodifiedtodisplayaproteinorpeptideasafusiontothecoatproteinpIII.OnlygenesIIIandVIIIofthephageDNAareshownasthesearethemostutilizedfordisplaypurposesandintheselectionofpeptidestoinorganicmaterials.Allfivephagecoatproteinsarealsolabeled.(b)?RepresentationofM13phagewithnanoparticles(blackspheres)nucleatedontopIII(darkblue).Aninorganicbindingpeptideisdisplayed(notshown)oncoatproteinpIIIandusedtonucleatenanoparticlesofthematerial.?(c)?SchematicofM13phageusedasatemplatefortheconstructionofone-dimensionalstructures.Inthiscase,ametal-orsemiconductor-bindingpeptideisdisplayedoncoatproteinpVIIIandagainisusedtonucleatenanoparticles(redspheres).Thisstructurecanbeannealedtoformawireortheendscrosslinkedtoformaring.Notethatparts(b)and(c)aredrawntodifferentscales.Fig.2.CellsurfacedisplaysystemsinGram-negativebacteria;greencirclesrepresentheterologouspassengerproteins.(a)SurfacedisplaysystemsdevelopedinGram-negativebacteria.Thepatentsdescribingthesedisplaysystemsareasfollows:S-layerprotein(US5874267),OmpC(US6274345),PhoA(US535697),OprF(WO9324636),OmpA(DE4243770,EP0474891),lipoprotein(WO950479),IgAprotease(WO9735022),Pilin(WO9410330),Lpp–OmpA(WO9310214,WO9849286),INP(WO9737025,WO9967366,WO0246388)andFlagella(WO006010).(b)Cell-surfacedisplaysystemusingicenucleationprotein(INP),whichisarepresentativeexampleoftheN-terminalfusionmethod.TheINPisthemoststableandusefulcarriertoexpressforeignproteinsaslargeas60kDa.(c)Cell-surfacedisplaysystemusingE.colioutermembraneproteinC,whichisarepresentativeexampleofsandwichfusionmethod.Inthissystem,poly-histidine(poly-His)peptidesofupto162