水稻雌性败育基因FA的图位克隆.pdf

中国水稻科学(ChinJRiceSci),2018,32(6):519－528

DOI:10.16819/j.1001-7216.2018.8023519

水稻雌性败育基因FA的图位克隆

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吕凤唐倩莹王启明郑海尤世民柏文婷肖晏嘉赵志刚万建民

12

(南京农业大学作物遗传与种质创新国家重点实验室，南京210095；中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学

*

工程,北京100081；通讯联系人，E-mail:zhaozg@)

Map-basedCloningofFemaleAbortion(FA)GeneinRice

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LÜFeng,TANGQianying,WANGQiming,ZHENGHai,YOUShimin,BAIWenting,XIAOYanjia,

ZHAOZhigang1,*,WANJianmin1,2

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(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity,Nanjing210095,China;NationalKeyFacilityfor

CropGeneResourcesandGeneticImprovement,InstituteofCropScience,ChineseAcademyofAgriculturalSciences,Beijing100081,China;*Corresponding

author,E-mail:zhaozg@)

Abstract:【Objective】Phenotypicobservation,geneticmappingandgenecloningofriceembryosacmutantswilllaya

theoreticalbasisforrecoveringplantreproductivedevelopment.【Method】Afemaleabortion(fa)mutantwasobtained

fromethylmethylsulfonate(EMS)-treatedNingjing4.Theembryosacsofthewild-typeandmutantwereobservedat

differentdevelopmentalstages,andthetypesofmutantembryosacswerealsocalculated.Theheterozygousmutantwas

usedasthefemaleparentandN22asthemaleparenttoconstructthemappi