着丝粒蛋白U在结直肠癌患者肠组织中的表达情况及临床意义.docx

着丝粒蛋白U在结直肠癌患者肠组织中的表达情况及临床意义

王若淳黄伟葛思佳陈婧宣晗颜杨姜佳炜肖明兵陆翠华刘肇修

[关键词]?结直肠癌；着丝粒蛋白U；富集分析

[中图分类号]?R735??????[文献标识码]?A????[DOI]?10.3969/j.issn.1673-9701.2024.11.001

Expression?and?clinical?significance?of?CENPU?in?intestinal?tissues?of?patients?with?colorectal?cancer

WANG?Ruochun1，2，?HUANG?Wei1，?GE?Sijia1，?CHEN?Jing1，2，?XUAN?Han1，2，?YAN?Yang1，2，?JIANG?Jiawei1，2，?XIAO?Mingbing1，2，???LU?Cuihua1，?LIU?Zhaoxiu1

1.Department?of?Gastroenterology，?Affiliated?Hospital?of?Nantong?University，?Nantong?226001，?Jiangsu，?China;?2.Clinical?Medical?Research?Centre，?Affiliated?Hospital?of?Nantong?University，?Nantong?226001，?Jiangsu，?China

[Abstract]?Objective?To?investigate?the?expression?of?centromere?protein?U?（CENPU）?in?the?intestinal?tissues?of?patients?with?colon?cancer，?and?to?analyze?the?effect?of?CENPU?expression?level?on?the?prognosis?of?patients?with?colon?cancer?combined?with?bioinformatics.?Methods?Firstly，?the?expression?of?CENPU?in?cancer?tissues?and?normal?tissues?of?colon?cancer?patients?was?analyzed?by?the?expression?of?CENPU?in?tissues?was?further?verified?by?real-time?quantitative?real?time?polymerase?chain?reaction?（qRT-PCR），?Western?blot?（WB）?and?immunohistochemistry?（IHC）.?Combined?with?clinical?data，?univariate?and?multivariate?Cox?regression?are?used?to?analyze?the?correlation?between?CENPU?expression?and?clinical?case?parameters?of?colon?cancer?patients.?Then，?the?predictive?effect?of?CENPU?expression?on?the?prognosis?of?colon?cancer?patients?are?explored?by?drawing?receiver?operating?characteristic?（ROC）?curve?and?Kaplan-Meier?survival?curve.?Finally，?the?possible?molecular?mechanism?of?the?effect?of?CENPU?expression?on?the?progression?of?colon?cancer?are?analyzed?by?bioinformatics.?Results?By?qRT-PCR，?WB?and?IHC?experiments，?we?find?that?compared?with?normal?tissues，?the?expression?of?CENPU?in?cancer?tissues?of?colon?cancer?patients?is?significantly?increased.?Cox?regression?analysis?show?that?the?expression?of?CENPU?is?significantly?correlated?with?th