转录因子CEBPB激活FJX1促进结肠癌细胞的增殖、侵袭、迁.docx

转录因子CEBPB激活FJX1促进结肠癌细胞的增殖、侵袭、迁移和血管生成能力的研究

滕向龙朱锡元邹武军

[摘要]?目的?探究CCAAT增强子结合蛋白B（CCAAT?enhancer?binding?protein?beta，CEBPB）与四连接同源基因（four-jointed?box?kinase?1，FJX1）在结肠癌（colon?cancer，CC）中的调控关系及其对CC恶性进展及血管生成的影响。方法?通过生物信息学分析FJX1和CEBPB在CC中的表达情况及二者之间的调控关系。利用实时荧光定量核酸扩增检测系统（real-time?quantitative?reverse?transcription?polymerase?chain?reaction，qRT-PCR）验证FJX1与CEBPB在CC细胞中的表达情况，利用染色质免疫沉淀（chromatin?immunoprecipitation，CHIP）与双荧光素酶实验验证FJX1与CEBPB之间的结合关系。利用細胞计数试剂-8（cell?counting?kit-8，CCK-8）、划痕试验、Transwell和血管形成实验检测FJX1与CEBPB对CC细胞增殖、迁移、侵袭及血管形成能力的影响。结果?本研究发现FJX1在CC中高表达，抑制FJX1的表达会显著抑制结肠癌细胞的细胞增殖、迁移、侵袭及血管形成能力。CEBPB是FJX1的上游调控基因，且CEBPB在结肠癌中高表达。CHIP与双荧光素酶实验结果显示，CEBPB可与FJX1相结合。细胞实验结果表明，转录因子CEBPB可通过激活FJX1的表达，从而促进CC细胞的增殖、迁移、侵袭及血管生成。结论?CEBPB/FJX1轴在CC的进展中发挥促癌作用，提示CEBPB和FJX1可能是CC的潜在治疗靶点。

[关键词]?结肠癌；CCAAT增强子结合蛋白B；四连接同源基因；血管生成；细胞增殖

[中图分类号]?R735????[文献标识码]?A????[DOI]?10.3969/j.issn.1673-9701.2024.03.009

The?research?of?transcription?factor?CEBPB?activates?FJX1?to?promote?the?proliferation，?invasion，?migration?and?angiogenesis?of?colon?cancer?cells

TENG?Xianglong，?ZHU?Xiyuan，?ZOU?Wujun

Department?of?Anorectal?Surgery，?Lishui?People’s?Hospital，?Lishui?323000，?Zhejiang，?China

[Abstract]?Objective?To?explore?the?regulatory?relationship?between?CCAAT?enhancer?binding?protein?beta?（CEBPB）?and?four-jointed?box?kinase?1?（FJX1）?in?colon?cancer?and?their?effect?on?colon?cancer?（CC）?malignant?progression?and?angiogenesis.?Methods?Bioinformatics?was?used?to?analyze?the?expression?of?FJX1?and?CEBPB?in?CC?and?the?regulatory?relationship?between?them.?Real-time?quantitative?reverse?transcription?polymerase?chain?reaction?（qRT-PCR）?was?used?to?verify?the?expression?of?FJX1?and?CEBPB?in?CC?cells，?and?chromatin?immunoprecipitation?（CHIP）?and?dual?luciferase?assay?were?used?to?verify?the?binding?relationship?;between?FJX1?and?CEBPB.?The?effects?of?FJX1?and?CEBPB?on?the?viability，?migration，?invasion?and?angiogenesis?of?CC?cells?were?detected?by?cell?counting?kit-8?（CCK-8），?scratch?test，?Transwell?and?angiogenesis?test.?