DNAProtein structure-function analysis and prediction：DNA蛋白质结构与功能的分析与预测.ppt

Bioinformatics Master Course:DNA/Protein Structure-Function Analysis and Prediction Lecture 13: Protein Function Systems Biology is the study of the interactions between the components of a biological system, and how these interactions give rise to the function and behaviour of that system (for example, the enzymes and metabolites in a metabolic pathway). The aim is to quantitatively understand the system and to be able to predict the system’s time processes the interactions are nonlinear the interactions give rise to emergent properties, i.e. properties that cannot be explained by the components in the system Biological processes include many time-scales, many compartments and many interconnected network levels (e.g. regulation, signalling, expression,..) Systems Biology understanding is often achieved through modeling and simulation of the system’s components and interactions. Many times, the ‘four Ms’ cycle is adopted: Measuring Mining Modeling Manipulating The citric-acid cycle The citric-acid cycle The citric-acid cycle Experimental Structural genomics Functional genomics Protein-protein interaction Metabolic pathways Expression data Communicability: Functional Genomics Interpretation of genome-scale gene expression data Communicability: Functional Genomics Interpretation of genome-scale gene expression data Other Issues Partial information (indirect interactions) and subsequent filling of the missing steps Negative results (elements that have been shown not to interact, enzymes missing in an organism) Putative interactions resulting from computational analyses Protein function categories Catalysis (enzymes) Binding – transport (active/passive) Protein-DNA/RNA binding (e.g. histones, transcription factors) Protein-protein interactions (e.g. antibody-lysozyme) (experimentally determined by yeast two-hybrid (Y2H) or bacterial two-hybrid (B2H) screening ) Protein-fatty acid binding (e.g. apolipoproteins) Protein – small molecules (drug interaction, struct