PRRSV美洲型经典株、变异株和疫苗株多重RT-PCR鉴别检测方法的建立及应用.pdf

2017 44 3 879887 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j P R RSV RTP CR orcine reproductive and respiratory syndrome virus PRRSV p PRRSV T JM F N sp PRRSV RT PCR L PRRSV T JM F T JM F PRRSV T JM F RT PCR PRRSV RT PCR S A Establishment and Application of a Multiplex RTPCR Assay for Differential Detection of Classical Highly Pathogenic and Vaccine Strains of North American Genotype PRRSV SHI Kaichuang XU Xinting HU Jie SU Yan iong LU Wenun CHEN Hanzhong q j Guangx i Center f or A nimal Disease Control and Prev ention N anning China College of A nimal Science and Technology Guangx i Univ ersity N anning China Abstract T o establish a rapid method f or diff erential detection of classical highly pathogenic and T JM F vaccine strains of North A merican genotype porcine reproductive and respiratory sy n drome virus PRRSV a multiple RT PCR assay w as established In this assay tw o pairs of primers w ere designed according to the genomic sequences of classical highly pathogenic and T JM F vaccine strains of PRRSV T he assay could only detect PRRSV but not detect CSFV FM DV PRV and PCV T he detection limit of the method w as as little as copies L of templates T he established assay w as successf ully used to detect clinical samples and samples w ere po sitive f or PRRSV of w hich samples w ere pos