猪卵母细胞在GV期与MⅡ期的冷冻保存效果比较研究.pdf

2017 44 3 807812 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j GV M GV M GV M h Western blotting CD GV h M P P M CD P GV P GV M P GV M CD CD S A Comparative Effects of Porcine Oocytes Vitrified at the GV and M Stages WU Guo uan QUAN Guobo LV Chunrong SHAO Qing ong HONG Qionghua q y Yunnan Prov incial Engineering L aboratory of A nimal Genetic Resource Conserv ation and Germp lasm Enhancement Yunnan A nimal Science and Veterinary Institute K unming China Abstract T his study w as designed to compare the efficiency of porcine oocy tes vitrified at the GV and M stages T he vitrified GV oocy tes w ere matured in v itro and then evaluated their cortical ranule distribution w ith immunof luorescence CD rotein level w ith Western blotting and zona g p pellucida dissolution time w ith pronse digestion method as compared to vitrified M oocy tes T he result s show ed that the survival percentages of oocy tes vitrified at the GV stage w ere sig nifi cantly low er than those vitrified at the M stage af ter h of w arming P How ever there w as no diff erence in maturation rate betw een vitrif ied and f resh oocy tes P T he oocy tes vitrified at M stage resulted in signif icantly decreased normal cortical granule distribu tion and CD rotein level P but no such result s w ere f ound in vitrified GV oocy tes af p ter maturation P In addition vitrification did not aff ect the dissolution time of zona pellucida in GV and M oo