丁香叶总酚酸提取工艺优化.doc

东北农业大学学士学位论文 学号： 丁香叶总酚酸提取工艺Dissertation for the Bachelor Degree of NEAU Student Number: A060100127 Optimization of extraction process of total phenolic acids from Syringa oblate Lindl Leaves. Candidate:Hu Zhiguang Supervisor: Pro. Li Yanhua Faculty: College of Veterinary Medicine Specialty: Veterinary Medicine Research Direction: Veterinary Medicine Northeast Agricultural University China · Harbin 2014-5 摘 要 丁香叶是一种理想的广谱抗菌药响应面法对丁香叶总酚酸乙醇提取工艺进行优化。总酚酸提取率为单因素试验乙醇浓度、提取温度、提取时间和液料比乙醇浓度44%、提取温度90℃、提取时间96min、液料比25.21(mL/g)、提取次数为2次，在此条件下提取率为1.64%，与预测值1.66接近。本研究表明该工艺合理可靠。 关键词:丁香叶；总酚酸； 响应面；提取工艺 Optimization of extraction process of total phenolic acids from Syringa oblate Lindl Leaves. Abstract Syringa oblate Lindl leaves is an ideal broad-spectrum antimicrobial drugs.The total phenolic acid compounds as the material basis of pharmacodynamic action, but the extraction rate is too low, in order to increase the extraction rate of total phenolics, the total phenolics of clove leaf ethanol extraction process was optimized by response surface methodology.Response surface methodology (RSM), based on a three level, four variable Box-Behnken design (BBD), was employed to obtain the best possible combination of ethanol concentration (A:20-40%), extraction temperature (B: 80–100 ?C), extraction time (C: 90–110 min), and water to the raw material ratio (D: 20–30) for maximum total phenolic acids extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: ethanol concentration of 44%, extraction time of 96 min, extraction temperature of 90 ?C, and the ratio of water to raw material of 25.2. Under these conditions, the experimental yield was 1.64±0.23%, which is well in close agreement with the value pre