姜黄的药理作用.ppt

Hepatic protection and anticancer activity ofcurcuma Content Introduction Materials and methods Result Introduction Turmeric is Curcuma Genus turmeric plant (Curcuma longa L.) dry roots. Modern pharmacological studies have shown that turmeric has hepatic protection and anticancer activity. Con A-induced hepatic injury model Male C57 mice were divided into 3 groups: Con A + curcuma oil group : pretreated with curcuma oil at 100 mg/kg (i.p.) for 3 days followed by a treatment of Con A at 30 mg/kg (i.v., tail vein) Con A group : pretreated with saline (same volume as curcuma oil, i.p.) for 3 days and then treated with Con A at 30 mg/kg (i.v., tail vein) The control group : treated with saline only Orthotopic mouse liver cancer model Eight-week-old C57L/J mice were housed fie per cage, given standard commercial chow (20) and tap water and maintained on a 12-h light/dark cycle. Hepa1-6 cells were maintained at 37?C, 95% air and 5% CO2 in Dulbeccos modifid Eagles medium (DMEM), supplemented with 10% FBS. The Hepa1-6 cells were injected into the right flnk of C57L/J mice at 1x107 in 100 μl. When the tumors were reached a size of ~1,000-2,000 mm3, the tumor cells were isolated for cell culture. The 3-4 generation of subcultured Hepa1-6 cells were used to established orthotopic growth liver cancer in C57L/J mice. The cells were directly inoculated into the left liver lobe of mice at 1x106 through a 27-gauge needle. Twenty-five mice were divided into three groups: Control group Tumor inoculation group Tumor inoculation + curcuma oil group The mice were pretreated with curcuma oil every 3 day for 1 week before Hepa1-6 cell inoculation and treated curcuma oil every 3 day after Hepa1-6 cell inoculation, at does 100 mg/kg (i.p.). The same volume saline was administrated as controls. Histopathology, cancer incidence and tumor weight Assays for in vitro cell viability and apoptosis Western blot analysis Result Thanks ! * *